Abstract: A method for determination of the four metabolites of nitrofuran antibiotics such as 3-amino-2-oxazolidinone (AOZ), 3-amino-5-morpholinomethyl-2- oxazolidinone (AMOZ), semicarbazide (SEM) and 1-aminohydantoin(AHD) in Poultry Tis- sues by LC-MS-MS was developed. Poultry Tissues samples was hydrolyzed with 0.2M HCl, and derivatized at 37℃ for 16h with 2-nitrobenzaldenhyde. The derivatized solutions were adjusted to pH=7.4, and then were cleaned up with LiChrolut EN cartridge, the target compounds were eluted with ethyl acetate and diluted with mobile phase. MS detection was performed in the positive ion mode using multiple reaction monitoring (MRM). The standard calibration curve for four kinds of metabolites were obtained through the synchronized operation of using blank Poultry Tissues sample fortification standard and Poultry Tissues samples for a quantitative analysis per the external standard. In the range of fortification concentration of 0.5～5μg/kg, the average recoveries for these four kinds of metabolites fell within 70.2%～89.1%. Limit of detection for AOZ and AMOZ was 0.1μg/kg, limit of detection for SEM and AHD 0.2μg/kg. The relative standard deviation (CV) was between 5.59%～10.10% (n=10).

Key words: LC-MS-MS, nitrofuran antibiotics, poultry tissues