FOOD SCIENCE ›› 2005, Vol. 26 ›› Issue (12): 196-198.

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Improved Determination Method of Free Amino Acids in Soy Sauce by Capillary Electrophoresis with Indirect Ultraviolet Detection

 FU  Chong-Gang, YANG  Dong-Zhi, WANG  Li-Xin   

  1. 1.School of Chemistry and Chemical Engineering, Liaocheng University; 2.School of Educational Technology and Mass Medium, Liaocheng University
  • Online:2005-12-15 Published:2011-10-01

Abstract: Byc apillarye lectrophoresisw ithi ndirectu ltravioletd etection, sixa minoa cids, namely alanine, serine, valine, leucine, glutamic acid and aspartic acid were detected in Tris (pH=10.50) where benzoic acid was taken as its setting electrolyte. Under the optimum conditions, these six amino acids were well separated within 5 minutes. The relative standard deviation (RSD) of the method was better than 5%. The linear calibration range was 10~1000μmolL and the detection limit were 2.0, 3.5, 4.0, 5.0, 3.5 and 5.0μmol L respectively. Their recoveries were all in the range of 95.73%~104.46%.

Key words: capillary electrophoresis, indirect ultraviolet detection, amino acid, sauce