FOOD SCIENCE ›› 2005, Vol. 26 ›› Issue (3): 66-68.

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A Simplified Method for Extracting DNA from the Mycelium of Edible Fungi

 ZANG  Jin-Ping, LIAN  Bin, YUAN  Sheng   

  1. Key Laboratory of Microbial Engineering, College of Life Sciences, Nanjing Normal University
  • Online:2005-03-15 Published:2011-09-19

Abstract: The DNA of edible fungi was directly extracted from fresh mycelium growing on the solid medium. PCR was performed with total DNA as a template at an annealing temperature of 56℃;Positive amplification was obtained from the mycelium of edible fungi. The result indicated that the method was simple, easy and reliable for DNA extraction from the mycelium of edible fungi cultured on solid medium.

Key words: DNA extraction, edible fungi, PCR