FOOD SCIENCE ›› 2005, Vol. 26 ›› Issue (4): 156-159.

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Study on Extraction Technology of Trifolium pratense Isoflavones

 CHEN  Han-Qing, JIN  Zheng-Yu   

  1. School of Food Science and Technology, Southern Yangtze University
  • Online:2005-04-15 Published:2011-09-19

Abstract: Extraction technology of isoflavones in Trifolium pratense was studied by single factor and orthogonal experiment. Effects of ethanol-water solvent, extraction temperature, extraction time, extraction times and ratio of liquid to material on extraction rate of isoflavone were investigated. The optimum extraction conditions for Trifolium pratense isoflavones were established as follows: 80% ethanol (V/V) as solvent, ratio of solvent to material 15:1(V/W), reflux extraction at 80℃ for 3 times and 1h for each time. The extraction rate of isoflavones in Trifolium pratense was as high as 91.90%.

Key words: Trifolium pratense, isoflavone, orthogonal experiment