FOOD SCIENCE ›› 2005, Vol. 26 ›› Issue (4): 55-58.

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Studies on Inoculation Types of γ-linolenic Acid Fermentation by a High-producing Filamentous Fungus Strain

 DAI  Chuan-Chao, SHI  Yang, WANG  An-Qi, YUAN  Zhi-Lin   

  1. College of Biology Science, Nanjing Normal University
  • Online:2005-04-15 Published:2011-09-19

Abstract:  A filamentous fungi strain identified as Rhizopus sp., producing high γ-linolenic acid (GLA) was obtained. In its mycelium, GLA amounted to 12.85% of the fatty acid while oil amounted to 19.70% of the mycelia. Two inoculation types were compared. By the spore inoculation the biomass, GLA composition and oil content of the mycelia were respectively 4.17g/L, 18.41% and 28.34%. By using mycelia inoculation gots the results were 25.98%, 51.90%, 43.87% higher than those above respectively. Using the mycelium as the secondary inoculums the final GLA content could reach the same level as that of the spores’; but the biomass could not meet the level of the spore inoculation.

Key words: γ-linolenic acid, Rhizopus sp., spore inoculation, mycelia inoculation, liquid seed