Purification and Characterization of Glucoamylase from Rhizopus oryzae

Abstract

Abstract: Glucoamylase is a kind of extracellular enzyme of Rhizopus oryzae induced in amylum culture medium. Its expression has a great impact on the production efficiency of L-lactic acid and the length of L-lactic acid batch fermentation cycle by Rhizopus oryzae. Purification and characterization of glucoamylase from Rhizopus oryzae were carried out in this dissertation. The results indicated that ammonium sulphate fractional precipitation, dialysis and Sephadex G-100 gel filtration could purify the glucoamylase from the extract of Rhizopus oryzae and increase its specific activity by 23 times. The SDS-PAGE showed that the purified enzyme was a homogeneous protein with molecular weight around 75.5kD. The optimum reaction pH value and temperature was about 4.0～6.0 and 40～50℃, respectively. It was stable under 50℃. The glucoamylase was activated by Ca 2+ and Mn2+,but inhibited by Pb2+ and Fe2+.

Key words: Rhizopus oryzae, glucoamylase, purification, characterization

PAN Li-Jun, ZHANG Zhi-Ying, JIANG Shao-Tong, ZHENG Zhi, LUO Shui-Zhong, HU Wei-Sheng. Purification and Characterization of Glucoamylase from Rhizopus oryzae[J]. FOOD SCIENCE, 2005, 26(6): 27-30.

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Purification and Characterization of Glucoamylase from Rhizopus oryzae

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