Synthetic Study of Immunogen Sulphadimine Residue in Edible Foods

Abstract

Abstract: In this paper, artificial haptene was synthesized taking succinic anhydride as spacer arm. Thin-Layer chromatographyand high performance liquid chromatography were applied to determinate it. Then, Reactive Ester Method was used tosynthesis complete antigen. After the level of protein measured with Bradford Method, conjμgated ratio was estimatedassociated with ultraviolet spectroscopy. The results indicated that the purity of hapene was high with the determination ofultraviolet spectroscopy and the conjugated ratio of BSA and OVA were l5 and 4 respectively.

Key words: SAs, ELISA, sulphadimine, active ester, veterinary drug residues

XU Chuan-Lai, PENG Chi-Fang, HAO Kai, LIU Li-Qiang, JIN Zheng-Yu, WANG Wu-Kang. Synthetic Study of Immunogen Sulphadimine Residue in Edible Foods[J]. FOOD SCIENCE, 2005, 26(7): 118-121.

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Synthetic Study of Immunogen Sulphadimine Residue in Edible Foods

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