Preparation of Aflatoxin B1 Artificial Antigen by EDC Method

FOOD SCIENCE ›› 2005, Vol. 26 ›› Issue (7): 125-128.

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Preparation of Aflatoxin B1 Artificial Antigen by EDC Method

JIANG Hu, XIONG Yong-Hua, XU Yang, HUANG Zeng-De

Sino-Germany Joint Research Institute, Nanchang University, The Key Laboratory of Food Science of MOE

Online:2005-07-15 Published:2011-09-19

Abstract

Abstract: Aflatoxin B1 artificial antigen was prepared with EDC method in this paper. Thin layer chromatography (TLC) andELISA were used to monitor the process for controlling the reaction during the preparation. The optimal conditions forpreparation were established in this study. The Aflatoxin B1 artificial antigen was obtained with the molar ratio of 5:1 when thesubstances were oximed for 24h at 25℃, and coupled for 24h as the initial molar ratio of Aflatoxin B1 to C-BSA was 30:1.

Key words: Aflatoxin B1, artificial antigen, EDC, oxime

JIANG Hu, XIONG Yong-Hua, XU Yang, HUANG Zeng-De. Preparation of Aflatoxin B1 Artificial Antigen by EDC Method[J]. FOOD SCIENCE, 2005, 26(7): 125-128.

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Preparation of Aflatoxin B1 Artificial Antigen by EDC Method

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