Selection of Phage-resistant Mutants from 2-keto-D-gluconic Acid Producing Strain Pseudomonas fluorescens A46

Abstract

Abstract: A phage, named KS461, was separated from the abnormal fermentation broth of Pseudomonas fluorescens A46 whichproduced 2-keto-D-gluconic acid. The KS461 was nearly spherical and 52nm in diameter as observed by transmission electronmicroscope. P. fluorescens A46 was mutagenized by using ultraviolet irradiation, and four phage-resistant mutants were obtainedwhich gave 2-keto-D-gluconic acid yield as high as that of the parent strain. Furthermore, the hereditary stability of the mutantswas investigated, and the results showed that the mutant resistance against the phage and 2-keto-D-gluconic acid producingability showed no change over 8 generations.

Key words: 2-keto-D-gluconic acid, Pseudomonas fluorescens, phage, phage-resistant mutant

SUN Wen-Jing, YANG Qing-Wen, ZHAO Feng-Mei, MA Hu-Ling, QIN Li, LIU Jing-Ze. Selection of Phage-resistant Mutants from 2-keto-D-gluconic Acid Producing Strain Pseudomonas fluorescens A46[J]. FOOD SCIENCE, 2005, 26(7): 67-70.

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Selection of Phage-resistant Mutants from 2-keto-D-gluconic Acid Producing Strain Pseudomonas fluorescens A46

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