Abstract: The cultivation condition affected the cell growth, the ice nucleation activity and the xanthan gum ratio. Through single factor test ,the best culture condition of Xanthomonas ampelina TS206 was determined. The optimum initial pH value was 7.0, the optimum broth capacity was 100ml, the optimum cultivation time was 48h .But the xanthan gum ratio could not be reduced effectively by the variation of the above mentioned culture condition. During the cultivation, changing temperature from 30℃ to 18℃ not only promoted the biomass and the ice nucleation activity but also inhibited the xanthan gum ratio and reduced the culture time obviously.

Key words: ice-nucleation active bacteria, xanthan gum, cultivation condition, two segment temperature culture