Abstract: In order to prepare Direct Vat Set (DVS) lactic-acid bacteria starter, the enrichment culture of Lactobacillus acidophilus ATCC 4356 was studied by orthogonality tests of carbon source, nitrogen source, nutrition factors and buffer salts. The optimum culture composition was as following: improved MRS culture,10% tomato juice,10% carrot juice,10% juice of plerotus sapidus, 0.1g/100ml CaCO3, 0.5g/100ml NaAc. The number of viable cells got to 8.56×109cfu/ml in the culture for 10 hours at 37℃. A notable effect of concentration was gained comparing with simple liquid starter.

Key words: Lactobacillus acidophilus ATCC 4356, enrichment culture, optimization