FOOD SCIENCE ›› 2005, Vol. 26 ›› Issue (9): 327-329.

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Fermentation Conditions for High-Level Expression of Trichosanthin Gene in Gene Engineering Strain

 ZHAO  Qiao-Yun, LI  Zhi-Dong, SONG  Ji-Rong, DONG  Zhao-Lin   

  1. 1.Department of Chemical Engineering;2.Department of Microbiology, Fourth Military Medical University;3.Department of Life Science, North-West University
  • Online:2005-09-15 Published:2011-09-20

Abstract: Objective: Engineering Strain(T239) was cultivated under different conditions in order to find the optimal Fermentations that would lead to maximal fusion protein yield. Methods: The strain was cultured under different temperature, pH, rotating rate, time, and capability of bottle Results and conclusions: The proper Fermentation Conditions consist of: 30℃, pH7.3,200r/min, 50ml/500mlculture bottle, 30h, rotating cultivation bed, with 2.0g/L glucoseon added.

Key words: Trichosanthin, geneengineering strain, fermentation