Detection of Genetically Modified Components in Maize and Its Products by Single PCR and Multiplex PCR

Abstract

Abstract: The total DNAs of maize and its products were extracted by CTAB method. The positive samples were screened by detecting of several genetically modified components such as Cauliflower mosaic virus(CaMV) 35S promoter, Agrobacterium tumefaciens nopaline synthase(nos) terminator, A.tumefaciens strain CP4 EPSPS gene, Treptomyces hygroscopicus bar gene and Bacillus thuringiensis subsp. kurstaki cryIA(b) gene with single PCR. The multiplex PCR methods detecting synchronously of maize endogenous zein gene and several genetically modified components were developed. The results showed that the multiplex PCR methods developed for detecting synchronously of endogenous gene and genetically modified components were practical and worthy of being popularized, and there is no commercial genetically modified maize in our country, but foreign commercial transgenic maizes were inpoured into fujian province.

Key words: maize, genetically modified component, PCR, multiplex PCR

SHAO Bi-Ying, CHEN Wen-Bing. Detection of Genetically Modified Components in Maize and Its Products by Single PCR and Multiplex PCR[J]. FOOD SCIENCE, 2005, 26(9): 380-384.

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Detection of Genetically Modified Components in Maize and Its Products by Single PCR and Multiplex PCR

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