FOOD SCIENCE ›› 2005, Vol. 26 ›› Issue (9): 426-429.

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Determination in Dairy and Development Change during Fermentation of Hippuric Acid

 XIE  Guo-Xiang, WU  Li-Li, SONG  Ru-Sen, YU  Jing, ZHANG  Cun-Zheng   

  1. 1.Nanjing Sanition Supervisor Office; 2.Nanjing Dairy Group Corporation Nanjing;3.Food Safety and Research Service Center, Jiangsu Province Agricultural Academy
  • Online:2005-09-15 Published:2011-09-20

Abstract: A HPLC method for determination of hippuric acid in dairy is established, and the development change of hippuric acid in plain yoghurt is studied. Hippuric acid can be well separated by RP-HPIC on ODS column and with methanol-water- acetic as mobile phase and UV detector at 254nm. The average recovery ranges from 96.2% to 104.4%; CV is 1.60%; the detection limit is 1.8ng and the detected concentration limit is 0.09mg/Kg. The reserch shows that hippuric acid in the raw milk of plain yoghurt can be converted to benzoic acid 3 hours after the inoculation of Lactic acid bacteria . The method for forecasting the content of benzoic acid during fermentation is of greater use value in controlling the content of benzoic acid in plain yoghurt.

Key words: benzoic acid, hippuric acid, determination method, yoghurt, development change