Abstract: In order to exploit an advanced beverage for the health of human beings’ throats from the protocorm-like bodies (PLBs) of medicinal Dendrobium Huoshanenes, the optimal conditions for PLBs culture are studied. On the basis of a single factor experiment, the orthogonal test is designed. Results showed that (1) 1/2MS was suitable for the growth of PLBs of Dendrobium Huoshanenes, (2) Combination of potato solution 100g/L, banana solution 30g/L, IBA 1.0mg/L and sucrose 30g/L in 1/2 MS was the best culture condition. When PLBs were cultured in optimized medium, proliferation of PLBs increased 12.56 times, and polysaccharide content was 8.30mg/gFW.

Key words: Dendrobium Huoshanenes, suspension culture in liquid, increase multiples, orthogonal test, optimize