Synthesis and Identification of the Antigen of OFLX and the Production and Purification of Its Monoclonal Antibodies

Abstract

Abstract: The Ofloxacin(OFLX) is coupled to BSA as an immunogen and to OVA as a coating antigen using the coupling agent EDC. The immunogen and coating antigen have been identified by the method of ultraviolet scanner and SDS-PAGE. Mono- clonal antibodies hybridoma cell against OFLX 7 strain have been gained and ascites have been purified by using a caprylic acid/ ammonium sulfate precipitation (CA-AS). Subclasscs of surified ascites was IgG1. Valency of antibody purified is higher than 1:128000, and the antibody wasn’t cross-reactivity with Danofloxacin, Lomefloxacin, Ciprofloxacin, Norfloxacin, Kanamycin, Gentamicin, Tylosin by ELISA. And the purified monoclonal antibody against OFLX shows a very high degree of sensitivity ands pecificity.

Key words: Ofloxacin, monoclonal antibodies, ELISA

YU Hai-Feng, Zhang-Sen, Wan-Zhong-Hai, Li-Xiao-Yan, TAN Jian-Hua. Synthesis and Identification of the Antigen of OFLX and the Production and Purification of Its Monoclonal Antibodies[J]. FOOD SCIENCE, 2006, 27(10): 223-228.

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Synthesis and Identification of the Antigen of OFLX and the Production and Purification of Its Monoclonal Antibodies

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