Separation and Identification of β,β-carotene Geometrical Isomers by C30-HPLC-PDA

Abstract

Abstract: The geometrical isomers of β,β-carotene were separated by HPLC equipped with a photodiode array (PDA) detector on a C30 column under conditions as below: stationary phase=YMCTM Carotenoid S-5 column (250×4.6mm); mobile phase A=acetonitrile-methanol-TCA(75:25:0.05, V/V); mobile phase B=MTBE-TCA(100: 0.05, V/V); linear gradient: B increased from 0 to 80%(V/V) in 20 minutes; flow rate: 1.0ml/min. PDA wavelength range: 260～700nm; sample injection volume=20 μl. According to their chromatographic behavior, spectral characters and geometrical transition catalyzed by iodine, 4 isomers of 13,13’Z-, 11,11’Z-, 9,9’Z- and all E-isomers were identified. Result from this study is going to be the base to quantify β,β-carotene geometrical isomers by C30-HPLC-PDA.

Key words: &beta, ,&beta, -carotene； geometrical isomer； C30 column；

HUI Bo-Di, Li-Jing, Pei-Ling-Peng, Zhang-Ling-Xiao, Zhang-Yan, Hu-Ya-Xin. Separation and Identification of β,β-carotene Geometrical Isomers by C30-HPLC-PDA[J]. FOOD SCIENCE, 2006, 27(10): 252-255.

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Separation and Identification of β,β-carotene Geometrical Isomers by C30-HPLC-PDA

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