Abstract: Objective: To establish a method for determining the content of the four lignans in extract of Schisandra chinensis and S.sphenanthera simultaneously. MethodSamples were extracted with alcohol and CO2-SFE. The contents of schisandrin,Wuweizi ester A,schisandrin A and schisandrin B could be determined simultaneously by dual wavelength TLC- Scanning (λS=220nm,λR=340nm) after the extracts were developed by n-hexane- ethyl acetate (8:3) on self-made GF254 plates. ResultThe calibration curves were linear in range of 0.44～1.54μg for schisandrin(R=0.9986),0.60～2.10μg for Wuweizi ester A(R=0.9960),0.52～1.82μg for schisandrin A(R=0.9997) and 0.48 ～1.68μg for schisandrin B(R=0.9962) respectively. The average recoveries(n=5) were 104.5% for schisandrin(RSD=4.0%),95.0% for wuweizi ester A(RSD=2.8%),96.1% for schisandrin A (RSD=2.6%) and 102.1% for schisandrin B(RSD=1.9%). All of the contents of four lignans in extracts of schisandra with SCF- CO2 are higher than that in extracts of schisandra with alcohol. Conclusion: This method was found to be simple,fast and accurate for simultaneously determining the content of the four lignans in extract of Schisandra chinensisl and S. sphenanthera.

Key words:  , TLCS； S.sphenanthera； Schisandra chinensis； lignans； SFE-CO2；