Abstract:  Based on the principle of enzyme inhibition,using the immobilized chicken liver esterase and a flow injection analysis (FIA) enzyme biosensor (developed by ourselves),this paper investigated the inhibiting power of several insecticides to the enzyme,and selected methamidophos as the standard pesticide for the quantitive determination. The standard curves of inhibition ratios-methamidophos content were set up corresponding to different initial enzyme activity. At last,the quantitive detection for cucumber and greengrocery added certain enhanced insecticides including methamidophos,dimenthoate,acetphate,carbaryl and malathion was performed,and the results showed that the establishment of the quantitive standard could be helpful to estimate the insecticides residue in the samples.

Key words:  , enzyme inhibition； quantitive standard； standard curve； insecticides residue；