Determination of Two Components in Crataegus cuneata Sieb.et Zucc.by HPLC-PAD

Abstract

Abstract: Adetermination method of ursolic acid and oleanolic acid in Crataegus cuneata Sieb. et Zucc. by reversed-phase high performance liquid chromatography was established and at the time UV spectrum of two components was detected by means of photodiode array detector(PAD). The chromatographic column,Nucleosil C18 (4.6mm×300mm,5μm),methanol- water (87: 13,V/V) mobile phase with 0.8ml/min flow rate,the detected wavelength (210nm),and the column temperature(26℃) were adopted. The calibration curve of ursolic acid was linear under the content of 0.1244～2.488μg,the correlation coefficient was over 0.9996.The average recovery of ursolic acid was 98.1%. The calibration curve of oleanolic acid was linear under the content of 0.1192～2.384μg,the correlation coefficient was over 0.9999. The average recovery of oleanolic acid was 101.2%. The method is simple,accurate,reproducible and has wider linear range. The method can be used to determine ursolic acid and oleanolic acid in Crataegus cuneata Sieb. et Zucc.

Key words: Crataegus cuneata Sieb.et Zucc, ursolic acid, oleanolic acid, photodiode array detector, HPLC, content determination

ZOU Sheng-Qin, Chen-Wu. Determination of Two Components in Crataegus cuneata Sieb.et Zucc.by HPLC-PAD[J]. FOOD SCIENCE, 2006, 27(11): 438-440.

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Determination of Two Components in Crataegus cuneata Sieb.et Zucc.by HPLC-PAD

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