Abstract: Four strains(QS29, NN22, QQ06, QQ05) of di-mon mating of Lentinula edodes were Zinc-enriched in the medium with Zn2+ content ranged from 0.05 to 0.3mg/ml. The optimization of cultivation conditions of the strain NN22 was selected, and primodium test of the Zinc-enriched strain NN22 was carried out in Zn2+ of PDY. The results showed that the highest Zn2+ content of the strain NN22 was obtained within 20 days at 25℃ and initial pH7.0 in 0.1mg/ml Zn2+ of PDY. Under the optimized cultivation conditions, Zn2+ content and polysaccharide content of the strain NN22 reached 9.26mg/g and 5.19mg/g, respectively. The phase of primodium formed of the Zinc-enriched strain NN22 was 19 days in 0.1mg/ml Zn2+ of PDY, which was reduced 6 days compared to the original strain.

Key words: Lentinula edodes, zinc-enriched, optimization of cultivation condition, primodium test