Extraction Study on Superoxide Dismutase from Lactarius deliciosus

Abstract

Abstract: This paper studied different extracting methods to extract the superoxide dismutase(SOD) from the fresh and dry sample of Lactarius deliciosus Gray and Lactarius hatsudak Tanake respectively. The orthogonal test was designed according to four factors and four levels. Through analysis of variance, the optium conditions of extracting SOD were obtained. The two Lactarius deliciosus were compared. Purification of SOD precipitated with ammonium sulfate and Sephadex G-100 column chromatography and two times with acetone and then the type of SOD was assayed among the purification products. Through the experiment it was found that the Lactarius deliciosus Gray contains 3 different SOD, but the content of Fe-SOD is comparatively high.

Key words: , Lactarius deliciosus Gray； superoxide dismustase； extraction and purifying； precipitate thea law two times witha cetone；

DONG Ai-Wen, HUANG Mei-E, TANG Ke-Hua, ZHANG Li-Ying. Extraction Study on Superoxide Dismutase from Lactarius deliciosus[J]. FOOD SCIENCE, 2006, 27(4): 78-82.

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Extraction Study on Superoxide Dismutase from Lactarius deliciosus

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