FOOD SCIENCE ›› 2006, Vol. 27 ›› Issue (8): 68-71.

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Study on Protoplast Formation and Regeneration of Pectinase Producing Bacterial ZH-g

 ZHU  Hong-Li, SONG  Ji-Rong, ZHANG  Jia, YANG  Bin-Bin, XU  Kang-Zhen, HUANG  Jie, YANG  Ming-Yan   

  1. 1.Institute of Life Science,Northwest University,Xi′an 710069,China;2.Institute of Chemistry Engineering,Northwest University,Xi′an 710069,China;3.Shaanxi Scidoor Hi-Tech Biology Co.Ltd.,Xi′an 710054,China
  • Online:2006-08-15 Published:2011-10-14

Abstract: The optimum conditions for preparation and regeneration of ZH-g protoplast were obtained after studying the effects of different factors on them.Under this circumstance-treating strains in log phase with concentration of 1.2mg/ml lysozyme for 2 hours,after pretreatment on the cells with 0.6μg/ml ampicillin,the protoplast formation is up to 92%,and regeneration reaches 50.7%.

Key words: pectinase, bacterial, protoplast formation, protoplast regeneration