FOOD SCIENCE ›› 2007, Vol. 28 ›› Issue (1): 159-160.

Previous Articles     Next Articles

Extraction and Separation of Glycoproteins from the Mycelia of Paecilomyces cicadae with Analysis Test

 TAN  Ai-Juan, LIANG  Zong-Qi, LIU  Ai-Ying   

  1. College of Life Science, Guizhou University, Guiyang 550025, China
  • Online:2007-01-15 Published:2011-12-31

Abstract: By means of degreasing, water-extracting, acetone-precipitating and dialyzing in distilled water, the total carbohydrate in the mycelial powder of Paecilomyces cicadae is tested as 34.79% by Phenol-Sulphuric Measure method and the protein content is 63.45% by Lowry. The glycoprotein possesses two absorbing peaks (PS1 and PS2) under ultraviolet spectrum as separated by Sephadex G-25. The protein content is 77.1% in PS1 and 53.02% in PS2, while the total carbohydrate is 21.11% in PS1 and 44.98% in PS2, respectively.

Key words: Paecilomyces cicadae, mycelia, total carbohydrate, glycoprotein, column chromatography