Extraction and Separation of Glycoproteins from the Mycelia of Paecilomyces cicadae with Analysis Test

Abstract

Abstract: By means of degreasing, water-extracting, acetone-precipitating and dialyzing in distilled water, the total carbohydrate in the mycelial powder of Paecilomyces cicadae is tested as 34.79% by Phenol-Sulphuric Measure method and the protein content is 63.45% by Lowry. The glycoprotein possesses two absorbing peaks (PS1 and PS2) under ultraviolet spectrum as separated by Sephadex G-25. The protein content is 77.1% in PS1 and 53.02% in PS2, while the total carbohydrate is 21.11% in PS1 and 44.98% in PS2, respectively.

Key words: Paecilomyces cicadae, mycelia, total carbohydrate, glycoprotein, column chromatography

TAN Ai-Juan, LIANG Zong-Qi, LIU Ai-Ying. Extraction and Separation of Glycoproteins from the Mycelia of Paecilomyces cicadae with Analysis Test[J]. FOOD SCIENCE, 2007, 28(1): 159-160 https://doi.org/

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Extraction and Separation of Glycoproteins from the Mycelia of Paecilomyces cicadae with Analysis Test

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