Study on Characteristics of Polyphenol Oxidase (PPO) in Coprinus comatus

Abstract

Abstract: The characteristics of polyphenol oxidase (PPO) of Coprinus comatus were studied by spectrophotometer, including its catalytic, characteristics the optimum temperature, the optimum pH, the thermostability of enzyme and so on. It was also studied the effects of the inhibitors EDTA-2Na, NaHSO3, ascorbic acid, and citric acid affecting the activity of PPO of Coprinus comatus. The results showed that the optimum conditions are: 45℃ and pH6.0 for PPO. However, the PPO is inactivated in 80℃ water for 5min, and is extincted in 95℃ water for 80s. Four kinds of inhibitors have different effects on PPO, and the ascorbic acid has the strongest effect, and next is citric acid, while NaHSO₃ and EDTA-2Na have the weakest effects with the order of PPO as ascorbic acid>citric acid>NaHSO₃>EDTA-2Na.

Key words: Coprinus comatus, PPO, characteristic, inhibitor

LI Jun-Lan, LI Yi-Hua, ZHAO Qiu-Ling, LIU Zhi-Fang, FENG Jiu-Hai. Study on Characteristics of Polyphenol Oxidase (PPO) in Coprinus comatus[J]. FOOD SCIENCE, 2007, 28(1): 187-191.

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Study on Characteristics of Polyphenol Oxidase (PPO) in Coprinus comatus

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