FOOD SCIENCE ›› 2007, Vol. 28 ›› Issue (10): 360-363.

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Primary Epitope Mapping of β-Lactoglobulin from Buffalo Milk

 LI  Xin, GAO  Jin-Yan, CHEN  Hong-Bing   

  1. 1.Key Laboratory of Food Science, Ministry of Education, Nanchang University, Nanchang 330047, China; 2.Sino-German Joint Research Institute, Nanchang University, Nanchang 330047, China; 3.Department of Food Science, Nanchang University, Nanchang 330047, China
  • Online:2007-10-15 Published:2011-11-22

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Abstract: To map the mimotopes of β-lactoglobulin from water buffalo milk, the specific rabbit antibody against buffalo β-lactoglobulin were used to pan the specific phage from a heptapeptide phage display library. The positive clones were identified by indirect ELISA and competitive ELISA. After fourth round enrichment , sixteen positive clones were obtained and five different clones peptide sequence were deduced by DNA sequencing. Compared with the known IgG epitopes of β-lactoglobulin, six epitopes have been identified, which are located at fragment 41～51(VYVEELKPIPE), fragment 51～64(EGDLEILLQKWENDE), fragment81～96(VFKIDALNE NKVLVLD), fragment 141～156(KALPMHIRLSFNPTQL), fragment127～136 (LNENKVLVLD) and fragment 145～159(MHIRLSFNPTQLEEQ respectively.

Key words: buffalo milk； &beta, -lactoglobulin； panning； epitope mapping； food allergy；