FOOD SCIENCE ›› 2007, Vol. 28 ›› Issue (11): 456-459.

Previous Articles     Next Articles

Determination of Organic Acids in Shanxi Ageing Vinegar by High Performance Liquid Chromatography

 吕Li-Hua , LIANG  Li-Rong, ZHAO  Liang-Qi   

  1. 1.Chemical Biology and Molecular Engineering Laboratory of Education Ministry, Shanxi University, Taiyuan 030006, China; 2.The Center for Disease Control and Prevention of Taiyuan, Taiyuan 030001, China
  • Online:2007-11-15 Published:2011-11-22

Abstract: An ion-exclusion HPLC method for rapid determination of 8 organic acids in Shanxi ageing vinegar was developed in this paper. 8 organic acids were separated by the use of PRP-X300 ion-exclusion column, and the optimal separation conditions are as follows: 0.05mmol/L H2SO4 as mobile phase, flow rate at 1ml/min, column temperature at room temperature, UV detector at 210 nm, and sample size as 5 μl. The RSD is 0.72%~5.47%, and the recovery rate is 95.1%~105%. The method has the advantages of rapid and simple operation, good selectivity and high accuracy.

Key words: ion-exclusion, Shanxi ageing vinegar, organic acid, HPLC