Study on Gold Immunochromatography Assay for Rapid Detection of Aflatoxin B1

Abstract

Abstract: To establish a rapid assay for detection of aflatoxin B1, gold immunochromatography assay(GICA)was studied. Colloidal gold marked coupled with monoclonal antibody against aflatoxin B1 was jet sprayed onto the glass fiber. Aflatoxin B1- BSA and anti-donkey anti-mouse immunoglobulins were jet-positioned onto a nitrocellulose membrane. Sample pad, gold jed pad, nitrocellulose membrane and absorbent paper were assembled and cut into detecting card, respectively. The results showed that the sentitive standard of rapid assay for aflatoxin B1 visual detection limit is 5 ng/ml and detection time within 10 min. Repeatability of strip is 100%. The assay gives no false positive and false negative results. So it is very simple and convenient to rapidly detect aflatoxin B1 on the spot.

Key words: aflatoxin B1, gold immunochromatography assay, strip, rapid detection

DENG Sheng-Liang, LAI Wei-Hua, XU Yang. Study on Gold Immunochromatography Assay for Rapid Detection of Aflatoxin B1[J]. FOOD SCIENCE, 2007, 28(2): 232-236.

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Study on Gold Immunochromatography Assay for Rapid Detection of Aflatoxin B1

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