Determination of Food Acrylamide with Solid Phase Extraction and High Performance Liquid Chromatography

Abstract

Abstract: The method for acrylamide determination in food was established. This method basing on high performance liquid chromatography (HPLC) is simple, rapid, accurate and reliable. The acrylamide was extracted with water as solution and purified with solid phase extraction from food, and separated on a C18 column with 5% methanol water solution as the mobile phase, and detected at 197nm. The calibration curve showed good linearity in the range of 0.05～10ng (R2=1) .The detection limit of acrylamide is 2.5ng/g. The standard recovery rate is 99.84%±1.55% and the coefficient of variation is 1.55%.The sausage application sample recovery rate is 92.34%±0.51%, and the coefficient of variation is 0.55%.

Key words: acrylamide, food, high performance liquid chromatography(HPLC), solid phase extraction technology

LI Ji-Ping, LIU Wen-Sen, GAO Hong-Wei, WANG Xing-Long, CHEN Yue-Ming, SONG Ri-Zhe. Determination of Food Acrylamide with Solid Phase Extraction and High Performance Liquid Chromatography[J]. FOOD SCIENCE, 2007, 28(2): 250-254.

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Determination of Food Acrylamide with Solid Phase Extraction and High Performance Liquid Chromatography

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