Abstract: Objective: To investigate the effects of the complexes of SIBC (soy isoflavones and bovine colostrum) on BMD(bone mineral density), and SOD(superoxide disnmtase), MDA(malonaldehyde) and GSH(glutathione) concentration of uterus in ovariectomized rats. Methods: The osteoporosis model was induced by extirpating uterus of rats and the rats were given SIBC for 3 months. The tibias bone density and contents of calcium, phosphorus and zinc and MDA and GSH concentrations of uterus were all assayed by spectrophotometry. SOD activity was assayed after the extract of uterus brought to 25% saturation with (NH4)2SO4. The isozyme of uterus SOD activity was analyzed by 8%～37% concentration gel gradient electrophoresis. Results: Compared with model group, SIBC groups significantly increase BMD and concentrations of calcium, phosphorus, zinc; decrease the concentration of MDA and increase the concentration of GSH in uterus. The SOD activity in the model group is lower than the control group. The specific activity of SOD both in the middle dose group and high dose group and activity of Mn-SOD in the middle dose group also increase after given SIBC. The activity of the isozyme markedly decreases in the model group, but after given SIBC, the activity of isozyme of middle dose group and low dose group markedly increase. The SOD-like activity of SIBC is 3492U/g. Conclusions: SIBC could increase BMD and prevent bone loss and could increase the anti-oxidation of uterus in OVX(ovariectomy) rats. It showed that SIBC has improvement on osteoporosis disease.

Key words: soy isoflavone, bovine colostrum, uterus, BMD, SOD, MDA