Abstract: One testing technology of determining 77 organophosphrous and carbamate pesticides residues in fruits and vegetables is developed. In this study, the samples are extracted with acetone and water (8+2). The extract is distributed with dichloromethane, and then is cleaned by passing through one gel permeation chromatography (GPC) column and one active carbon solid phase extraction (SPE) column in sequence. The eluate is condensed to a definite volume, and then determined and confirmed by gas chromatography - mass spectrometry(GC-MS) with external standard method. Ten vegetables and fruits including spinach and orange etc. are selected as samples. 0.05～2.00mg/kg of the 77 pesticides such as dichlorvos etc. are fortified into blank samples. The corresponding recoveries are 53.6%～124.8% and the precisions are 4.01%～24.90% and the limit of determination (LOD) are 0.01～0.100mg/kg.

Key words: pesticide residue, fruits and vegetables, gas chromatography - mass spectrometry(GC-MS)