Determination of Cholesterol in Foods by HPLC Method

Abstract

Abstract: HPLC method was established to determine cholesterol in animal foods.The final chromatographic conditions are:C18chromatographic column,diode-array detector,detector wave 205 nm,100% methane elution influent and flow rate 1.0ml/min.The relative standard deviations of this method is below 5% and the recovery is between 96%～102%.The satisfied results can be obtained under this chromatographic conditions,and the separation can be finished within 15 min.The results showed this new HPLC analysis method is both correct and fast enough to be used in assaying most foods containing cholesterol.

Key words: HPLC, cholesterol, food

LI Li, LIU Yu-Feng, TANG Hua-Cheng, LI Dong, WANG Jing, FANG Xiang, WU Fang-Di. Determination of Cholesterol in Foods by HPLC Method[J]. FOOD SCIENCE, 2007, 28(4): 289-291.

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Determination of Cholesterol in Foods by HPLC Method

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