Abstract: This study is to evaluate the effects of proso millet prolamin concentrate(PMP)on cholesterol metabolism in mice, by comparing the concentrations of serum total cholesterol(TC),high-density lipoprotein cholesterol(HDL-c),low-density lipoprotein cholesterol(LDL-c)and triglyceride(TG)of mice given diets containing PMP(supplemented with 1.83% Lys and 0.23% Trp),or casein or soy protein isolation(SPI)respectively.The mice fed with PMP supplemented with Lys and Trp showed that HDL-c concentration(p＜0.05)is the significantly highest and atherogenic index(AI)is the lowest without affecting the level of TC and TG among three groups.In vitro,the PMP can not suppress the activities of 3-Hydroxy-3-methylglutaryl- ccenayme,a(HMG-CoA)reductase.It means that the PMP concentration can not inhibit the synthesis of cholesterol directly. Perhaps it can adjust the serum cholesterol metabolism by other ways.

Key words: Proso millet prolamin, amino acid composition, cholesterol modulation, HMG-CoA reductase