FOOD SCIENCE ›› 2007, Vol. 28 ›› Issue (5): 301-304.

Previous Articles     Next Articles

Determination of Chlorogenic Acid of Potato Tuber by HPLC

 ZHANG  Jian-Hua, JIN  Li-Ping, XIE  Kai-Yun, QU  Dong-Yu   

  1. Institute of Vegetable and Flower,Chinese Academy of Agricultural Sciences,Beijing 100081,China
  • Online:2007-05-15 Published:2011-12-31

Abstract: Object:To establish a new HPLC method for determination of chlorogenic acid(CGA)in potato,and determine its content of CGA in five cultivars.Method:Orthogonal experiment for three factors:CH3OH concentration,ratio of solid and liquid and time of ultrasounic extraction.CGA was analyzed with C18column(250 mm×4.6 mm,5μm)and with detector at 327 nm.The mobile phase consisting HO-CH3OH-CH3COOH(20:80:2;30:70:2;40:60:2 respectively),CH3OH-0.4% H3PO4 (10:90).Results:The recovery of CGA is 97.5%,precision(RSD)3.2%(n=6);CGA extraction conditions are:70% CH3OH,ratio of solid and liquid=1:100,and ultrasounic extraction time 40 min with determined mobile phase H2O-CH3OH-CH3COOH=30: 70:2.

Key words: HPLC, potato, tuber, chlorogenic acid