Identification and Isolation of Major Allergens in Milk

Abstract

Abstract: Object:To isolate and identify the main allergens in the milk.Method:The protein components in skimmed milk and whey were analyzed by SDS-PAGE.The allergen proteins were purified by the combined of techniques saturated ammonium sulfate salimetry and DEAE iron-exchanged chromatography.The Balb/c mice were immumed with the extracts of milk,then the high titrations of specific IgE anti-serum were isolated for immunoblotting analysis.Results:Immunoblotting analysis indicated thatβ-Lg is the major component leading to cow milk allergy.Allergic mice models were established successfully.Conclusion: The major allergic components in milk were identified in this study,paving an experiment basis for the diagnoses and therapy of milk allergey as well as the preparation of non-allergen milk.

Key words: milk, food allergen, immunoblotting, DEAE iron-exchanged chromatography

MAO Lu-Tian, XIANG Jun-Jian, ZHANG Zai-Jun. Identification and Isolation of Major Allergens in Milk[J]. FOOD SCIENCE, 2007, 28(5): 305-308.

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Identification and Isolation of Major Allergens in Milk

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