Abstract: On the basis of the production of high F ratio（Fischer ratio）oligo-peptide（F ratio is 21.7）from corn gluten meal hydrolysis by multi-enzymes,the function of anti-fatigue of high F ratio oligo-peptide was appraised and its beverage was produced.The results indicated that comparing test groups with the control groups,the swimming times in the high-dosage groups and mid-dosage groups increase 54%（p＜0.01）and 38%（p＜0.05）respectively.Urea nitrogen contents decrease 18%（p＜0.01）and 12%（p＜0.05）respectively.Liver glucogen contents are 3.2 times（p＜0.001）and 2.3 times（p＜0.01）higher respectively.Muscle glucogen contents are 1.7 times（p＜0.01）and 1.4 times（p＜0.01）respectively.After swimming,the test groups compared with the control groups in 0 min and 15 min,the mice contents of lactates decreased 31%（p＜0.01）and 27% （p＜0.05）respectively.The function of anti-fatigue of high F ratio oligo-peptide is proved.The optimum formula of the beverage is A2B2C2（fruitjuice 5%,granulated sugar 8% and citric acid 0.15%）.The product meets the quality standards.

Key words: corn gluten meal, high Fratio oligo-peptide, anti-fatigue, function evaluation, beverage