FOOD SCIENCE ›› 2007, Vol. 28 ›› Issue (8): 160-164.

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Study on Optimization of Extraction Process of Ursolic Acid from Potentilla discolor Bge with Ultrasonic

 LI  Sheng-Hua, WU  Xian-Jin, YU  Jian-Ping, WANG  Li-Na, HU  Mei-Zhong   

  1. 1.Department of Bioengineering, Huaihua College, Huaihua 418008, China; 2.Institute of Biochemistry and Nutrient, Guizhou University, Guizhou 550025, China
  • Online:2007-08-15 Published:2011-10-24

Abstract: Objective: To screen optimum the extraction process of ursolic acid from Potentilla discolor Bge. Methods: ultrasonics extraction was used to extract. The optimum extraction process was confimed by the orthogonal test. The content of ursolic acid in Potentilla discolor Bge. was determined by HPLC. Results: The optimum conditions of ultrasonics extraction are as follow: ethanol concentration 75%, time 60 min, ratio of material to solution 1:60, and power of extraction 610 W. The effects of factors on yield decrease progressively in the following order: extracting time>ratio of material to solution>ethanol concentration>power of extraction. Conclusion: The best optimized process has a good repeatability confimatory experiment.

Key words: Potentilla discolor Bge., ursolic acid, extraction