Biopanning of Zearalenone Mimotope by Phage Displayed Peptide Bank

Abstract

Abstract: Objective: To obtain mimotope of zearalenone (ZEN) by phage displayed peptide bank. Methods: The anti-ZEN monoclonal antibody was used as target for biopanning from a heptapeptide phage display bank. The concentration of antibody was reduced step by step, and different blocking buffer was alternated every round. After 3 rounds of biopanning, 20 clones were randomly selected for DNA sequencing, and assayed by ELISA and ZEN competition experiments to indentify the postive clones. Results: Ten sequences are obtained, two of which are proved to be positive. Inhibition percentages are up 90% and the amino acid sequences are DAVILLM, HHCHWWH respectively. Conclusion: The phage display technique can be successfully applied to panning the mimotope of ZEN.The aquired phages may be used as the surrogate of the toxin to establish immunoassay.

Key words: phage display peptide library, zearalenone, mimotope, monoclonal antibody

HE Qing-Hua, LIU Ren-Rong, XU Yang. Biopanning of Zearalenone Mimotope by Phage Displayed Peptide Bank[J]. FOOD SCIENCE, 2007, 28(8): 241-243.

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Biopanning of Zearalenone Mimotope by Phage Displayed Peptide Bank

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