FOOD SCIENCE ›› 2007, Vol. 28 ›› Issue (8): 276-279.

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Affinity Purification of Rabbit IgG Against Buffalo’s β-lactoglobulin

 LI  Xin, CHEN  Hong-Bing   

  1. 1.Key Laboratory of Food Science, Ministry of Education,Nanchang University, Nanchang 330047, China; 2.Sino-German Joint Research Institute, Nanchang University, Nachang 330047, China
  • Online:2007-08-15 Published:2011-10-24

Abstract: In order to collect high purity rabbit antibody against buffola’s β-lactoglobulin, β-lactoglobulin was coupled into the medium of Sepharose 4B, and then the medium was eluted with 3 mol/L MgCl2. The results showed that electrophoretically pure of IgG is identified by SDS-PAGE, the protein content is 5.8 mg/ml, and the titer is 1:109 using ELISA. So the method established can be used to purify the specific IgG in rabbit serum.

Key words: &beta, -lactoglobulin; IgG; affinity chromatography;