Mutation Breeding of Producing AD Strain by Complex Mutation and Natural Capacity Identification

FOOD SCIENCE ›› 2007, Vol. 28 ›› Issue (9): 343-345.

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Mutation Breeding of Producing AD Strain by Complex Mutation and Natural Capacity Identification

YANG Ying, JIANG Shao-Tong, HU Jin-Yan, ZHAO Jun-Ping

1.College of Food and Biological,Hefei University of Technology,Hefei 230009,China;2.Department of Environment Engineering,Anhui Institute of Architecture,Hefei 230022,China

Online:2007-09-15 Published:2011-11-22

Abstract

Abstract: One of key factors,which hindered producing androst-4-ene-3,17-dione(AD)on industrial scale is that the yield of androst-4-ene-3,17-dione is low.The microorganism of Mycobacterium sp.BD696# was treated by complex mutagenesis of UV,60Co and UV+60Co ray irradiation.And AD transformation productivity was 39.2% higher than that of its parent strain and no androst-1,4-ene-3,17-dione was obtained.The fifth stain also had stable transformation productivity.The results showed that complex mutagenesis of UV+60Co ray irradiation is a good methed for mutation breeding.

Key words: mutation breeding, UV light, androst-4-ene-3,17-dione, bioconversion

YANG Ying, JIANG Shao-Tong, HU Jin-Yan, ZHAO Jun-Ping. Mutation Breeding of Producing AD Strain by Complex Mutation and Natural Capacity Identification[J]. FOOD SCIENCE, 2007, 28(9): 343-345.

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Mutation Breeding of Producing AD Strain by Complex Mutation and Natural Capacity Identification

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