FOOD SCIENCE ›› 2007, Vol. 28 ›› Issue (9): 395-397.

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Construction of Eukaryotic Expression Vectors for Antibacterial Peptide Japonicin II

 CHEN  Xiao-Ping, ZHAN  Dong-Ling, JIA  Hui-Wen, SHANG  Xue-Jiao, QIAN  Ai-Dong   

  1. College of Food Science and Engineering,Jilin Agricutural University,Changchun 130118,China
  • Online:2007-09-15 Published:2011-11-22

Abstract: The antibacterial peptide Japonicin II gene was designed and artificially synthesized by SOE.Antibacterial peptide Japonicin II gene was subcloned into eukaryotic expression vector pPICZαA,by using PCR and sequence analysis.It can be proved that all the tested clones contain Japonicin II gene,and pPICZαA-Jap is successfully constructed.

Key words: antibacterial peptide Japonicin II, SOE, eukaryotic expression vectors, construction