Comparison Study on Enzymatic and Alkaline Extractions, Isolation, Purification and Functional Properties of Tremella Polysaccharides

Abstract

Abstract: Extraction, isolation, purification and functional properties of Tremella polysaccharides were studied in the paper. The results showed that the enzymatic extraction method was better than the alkali extraction. The optimal conditions for enzymatic extraction are 1:60 of the sample to water ratio, 0.7% of Citrozyme Cloudy, 50 min of enzymatic hydrolysis and 60 min of extraction. A pure polysaccharides TPP2 can be obtained by DEAE-Sepharose Fast Flow and SephadexG-200 purification of Tremella polysaccharides. TPP2 shows the active capability in scavenging DPPH·. The sample thickness is 55 μg/ml in 50% scavenging rate (IC50).

Key words: , Tremella polysaccharides； extraction； enzyme； purification；

HUANG Xiu-Jin. Comparison Study on Enzymatic and Alkaline Extractions, Isolation, Purification and Functional Properties of Tremella Polysaccharides[J]. FOOD SCIENCE, 2008, 29(1): 134-136.

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Comparison Study on Enzymatic and Alkaline Extractions, Isolation, Purification and Functional Properties of Tremella Polysaccharides

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