Application of SRP-HPLC with Gradient Elution for Rapid Determination of Minocycline Residues in Milk

Abstract

Abstract: To quickly establish reverse phase high-performance liquid chromatography (RP-HPLC) method to determine residues of Minocycline in the milk, the gradient elution method was developed. Minocycline was separated on a sunfire C18 column (250mm×4.6mm, 5μm i.d.) with methanol-acetonitrile-0.01mol/L oxalic acid at the flow rate of 1.0 ml/min and detecting at the 355 nm within 8 min. The results showed that the method under the gradient elution is more sensitive and quicker with lower detection limit than that of the isocratic elution. The detection limit is 20μg/kg for Minocycline. The standard curve is obtained in the concentration range of 0.02～1.0μg/ml with the average recovery 99%. The lowest detection limit is 0.02μg/ml. The method established is simple, special, sensitive and reliable. It can be used to detect the residue of Minocycline and the pharmacokinctic parameters of Minocycline in milk.

Key words: RP-HPLC, Minocycline, gradient elution, solid phase extraction

LE Tao, SHE Yong-Xin, LIU Jiang-Ying, BI Ding-Ren. Application of SRP-HPLC with Gradient Elution for Rapid Determination of Minocycline Residues in Milk[J]. FOOD SCIENCE, 2008, 29(1): 270-272.

[1]	MENG Zhijuan, HUANG Yunxia, LI Yan, SUN Wenyi, WANG Dong, LI Qiang, FAN Sufang, ZHANG Yan. Rapid Determination of 17 Phthalate Acid Esters Residues in Vegetable Oils by Gas Chromatography-Electrostatic Field Orbitrap High Resolution Mass Spectrometry [J]. FOOD SCIENCE, 2021, 42(6): 298-304.
[2]	WANG Zhuo, CHEN Churui, XU Liqiang, ZHUANG Peirui, ZHOU Wensi, CUI Chun. Effects of pH on the Types and Taste Characteristics of Flavor Peptides in Soy Sauce Produced under Low Temperature Stress [J]. FOOD SCIENCE, 2021, 42(2): 60-65.
[3]	ZHANG Yicai, LIN Qinbao, CHEN Sheng, ZHONG Huaining, CHEN Yanfen, ZENG Ying, WU Zechun, LI Zhong. Quantitation and Source Analysis of Mineral Oil Hydrocarbons in Traymates [J]. FOOD SCIENCE, 2021, 42(12): 261-267.
[4]	GONG Bichuan, HU Qiuhui, SU Anxiang, XU Hui, LIU Jianhui, PEI Fei, YANG Wenjian. Preparation and Application of Dummy Template Molecularly Imprinted Polymer in the Detection of Triazines in Fruits and Vegetables [J]. FOOD SCIENCE, 2021, 42(12): 281-287.
[5]	LI Chaoyi, LI Long, ZHU Ying, WU Ting, DU Yiping. Spectroscopic Determination of Trace Nitrite in Water and Meat Following Solid Phase Extraction [J]. FOOD SCIENCE, 2021, 42(12): 309-314.
[6]	MA Junmei, HE Liangna, SUN Lei, WANG Dong, ZHANG Leilei, ZHANG Yan, KANG Wenyi. Determination of Five Antiviral Drug Residues in Chicken Meat and Eggs Using On-line Solid Phase Extraction Coupled to Liquid Chromatography-Tandem Mass Spectrometry [J]. FOOD SCIENCE, 2021, 42(10): 305-310.
[7]	ZHANG Yanxia, ZHAO Huinan, SUN Shanshan, XU Xiangjun, ZHENG Wenjing, XUE Xia, WANG Mingdong, LIU Yanming, ZHU Jianhua. Simultaneous Determination of Nine Phenolic Antioxidants in Vegetable Oil by Pass-through Solid Phase Extraction Cleanup and Ultra-high Performance Liquid Chromatography-Tandem Mass Spectrometry [J]. FOOD SCIENCE, 2021, 42(10): 322-329.
[8]	LI Ling, SU Xuesu, ZHANG Yaohai, JIAO Bining, LIU Jintao, DONG Jiali. Preparation and Application of Molecularly Imprinted Polymer in Solid Phase Extraction of Synephrine [J]. FOOD SCIENCE, 2020, 41(8): 45-51.
[9]	TIAN Jingsheng, ZHANG Zhanzhan, QIN Sinan, GAO Wenhui. Preparation and Application of Magnetic Molecularly Imprinted Polymer for the Detection of Bitertanol in Foods [J]. FOOD SCIENCE, 2020, 41(24): 325-332.
[10]	REN Nan, ZHAO Wentao, CHEN Chao, WU Yanchao, ZHANG Yingying, LI Yingying, GUO Wenping, FAN Wei, WANG Shouwei. Determination of Three Catecholamines in Livestock Meat by Solid Phase Extraction Combined with Ultra-high Performance Liquid Chromatography-Tandem Mass Spectrometry [J]. FOOD SCIENCE, 2019, 40(24): 300-305.
[11]	QIAN Shasha, YANG Yang, ZHANG Yuping, ZHUANG Yan, JI Hanxu, LI Ning, WU Xiaoxiao, ZHANG Chi. QIAN Shasha, YANG Yang, ZHANG Yuping, ZHUANG Yan, JI Hanxu, LI Ning, WU Xiaoxiao, ZHANG Chi [J]. FOOD SCIENCE, 2019, 40(20): 305-310.
[12]	ZHANG Kaijie, QIN Sinan, ZHAO Chunjuan, GAO Wenhui. ZHANG Kaijie, QIN Sinan, ZHAO Chunjuan, GAO Wenhui [J]. FOOD SCIENCE, 2019, 40(20): 338-345.
[13]	LIU Wenhui, DONG Yue, WANG Na, LI Shaozhen. Determination of Patulin in Apple Juice and Apple Beverage by Solid Phase Extraction-High Performance Liquid Chromatography [J]. FOOD SCIENCE, 2019, 40(12): 303-307.
[14]	PENG Yaoshan, LI Yongli, CHEN Ying, LI Jie. Determination of 16 Polycyclic Aromatic Hydrocarbons in Meat Samples by Gas Chromatography-Isotope Dilution Mass Spectrometry Approach [J]. FOOD SCIENCE, 2019, 40(12): 321-325.
[15]	LIU Zhenzhen, LI Rong, TANG Shuhua, HUANG Xianyong, ZHANG Wanjing, LI Yongjie, JIANG Zitao. Separation and Enrichment of Casein Phosphopeptides Using SiO2@TiO2 Composite [J]. FOOD SCIENCE, 2019, 40(11): 75-81.

Application of SRP-HPLC with Gradient Elution for Rapid Determination of Minocycline Residues in Milk

RichHTML

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments