Abstract: Objective: To explore the effect of brewing bifidobeer on immuniological function of mice. Methods: Mice were randomly assigned to 5 groups: the purified water control group, the ordinary beer control group and three brewing bifidobeer groups(drunk the purified water containing brewing bifidobeer 25,50,100 ml/kg bw). The weight of thymus and spleen, the phagocytic capability of celiac macrophage, the level of serolysin and the degree of cutaneous delayed type hypersensitivity of mice were evaluated. Result: The phagocytic capability of celiac macrophage, the level of serolysin and the degree of cutaneous delayed type hypersensitivity of high dose and medium dosage brewing bifidobeer groups increased significantly compared with control groups. Conclusion: Brewing bifidobeer can enhance the immuniological function of mice.