FOOD SCIENCE ›› 2008, Vol. 29 ›› Issue (10): 290-294.

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Effect of Ultra High Pressure Processing Combined with Nisin on Survival Rate of Bacillus subtilis AS 1.140

 ZENG  Qing-Mei, YANG  Yi, ZHAO  Yang-Nan, YIN  Yun-Xu   

  1. Engineering Research Center of Bio-process,Ministry of Education,Hefei University of Technology,Hefei 230009,China
  • Online:2008-10-15 Published:2011-12-08

Abstract: Objective:To investigate the effect ultra high pressure processing(UHPP) combined with nisin on B. subtilis AS 1.140. Method: All experiments were designed according to pentagonal concept of RSM. Result: The pressure and nisin concentration are the most significant factors affecting UHPP sterilization to Bacillus subtilis,and their significance order is pressure>nisin concentration; The regression equation (model) for UHPP sterilization to B. subtilis was established,and also verified to be good for prediction in the range of experimental conditions; Nisin can be more effective to synergize sterilization of UHPP to B. subtilis than temperature; The increase of sterilization rate (the change of log-circles) is higher in nisin concentration of 0.00~0.05mg/ml than in that of 0.50~0.15mg/ml.

Key words: ultra high pressure, Bacillus subtili, snisin, response surface methodology (RSM), optimization, prediction