Abstract: Objective: To determine flavonoids content in rape bee pollen by HPLC and analyze the effects of six cell wall-breaking techniques on flavonoids extraction from rape bee pollen. Methods: The HPLC conditions were as follows: reversed-phase C18 column as separation column,methanol:0.4% phosphoric acid (60:40) as mobile phase,detection wavelength 370 nm,quercetin and kaempferol as external standards and flow rate 0.5 ml/min.The adopted wall-breaking techniques were ultrasonic method,enzymolysis method,temperature difference method,temperature difference-ultrasonic method,temperature difference-enzymolysis method and integrated method of the threee single methods. Results: The flavonoids content of original rape bee pollen is 2.2447 mg/g by HPLC. The flavonoids contents of wall-broken rape bee pollen by the above-mentioned methods are higher 12.4%,8.3%,23.1%,25.4%,28.1% and 31.5% than 2.2447 mg/g respectively,and the wall-breaking rates of these methods are 52.66%,41.31%,76.94%,78.66%,83.13% and 86.8% respectively. Conclusion: The HPLC method can provide scientific indexes for developing functional components of rape bee pollen. The higher wall-breaking rate,the more flavonoids released from rape bee pollen.

Key words: rape bee pollen, flavonoids, wall-breaking, HPLC