Composition Analysis of Flavonoids from Corn Silk with Thin-layer Chromatography and Ultraviolet Spectrophotometry

Abstract

Abstract: The experiment aimed to analyze the main components of flavonoids from corn silk by using color reaction,thin-layer chromatography(TLC) and ultraviolet(UV) spectral scanning.Color reactions proved the extract from corn silk be to flavonoids,According to TLC results,the flavonoids from corn silk are mainly composed of luteolin,formononetin and apigenin.The results of UV scanning showed that the flavonoids of corn silk are mainly flavones and isoflavones.There are 5-hydroxy and 7-hydroxy in the structure of the flavones,and the B ring has the 3’-hydroxy and 4’-hydroxy.

Key words: corn silk, flavonoids, color reaction, thin-layer chromatography, UV scanning

YU Ting-Ting, LU Xiao-Xiang, LIAN Xi-Jun, ZHANG Yan-Qing. Composition Analysis of Flavonoids from Corn Silk with Thin-layer Chromatography and Ultraviolet Spectrophotometry[J]. FOOD SCIENCE, 2008, 29(11): 477-481.

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Composition Analysis of Flavonoids from Corn Silk with Thin-layer Chromatography and Ultraviolet Spectrophotometry

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