Abstract: The composition of drug culture medium and technology conditions that increase the yield of biomass for Ganoderma lucidum submerged culture were optimized. Uniform design was used to evaluate the crucial factors affecting the yield of biomass. In the following phase of the optimization process,L9(34) orthogonal test was applied to get the optimal fermentation conditions. By statistical analysis,the optimum composition of drug culture medium affecting the yield of the biomass were determined as follows:drug substance(Liuweidihuang pills) 10 g/L,soybean powder 50 g/L,corn powder 10 g/L,glucose 8.29 g/L,MgSO4 1.0 g/L,KH2PO4 1.0 g/L and VB1 20 to 40 mg/L and the optimal fermentation conditions are as follows:inoculum size 10%,ventilation volume 0.5m3/min,agitation speed 150 r/min and duration 144 h. Uniform design and orthogonal test simultaneously used in the optimization of technology for Ganoderma lucidum fermentation in drug culture medium are proved to be effective.

Key words: Ganoderma lucidum, drug culture medium fermentation, optimization, Liuweidihuang pills, uniform design, orthogonal test