Abstract: The walnut septum was refluxed twice for two hours each with 100 ml 50% ethanol. With rutin as reference and boric citric acid was taken as developer, at 410 nm wavelength and the optimum conditions were determined by orthogonal design method. The flavone glycoside content in walnut benevolence is 0.15 mg/ml and recover rate 84%～114%.

Key words: walnut septum, flavone glycoside, boric acid-citric acid, orthogonal design method