Abstract: Five strains of Pleurotus eryngii from different provinces of China were primarily identified by antagonistic effects and then analyzed by RF-RAPD technology. The data showed that the results of RF-RAPD were consistent with those of antagonistic effects. Moreover, due to combining two molecular markers (RFLP and RAPD), RF-RAPD can effectively distinguish xiangxing PL-3 from jingxing PL-10 while otherwise they are closely related to each other. It was suggested that RF-RAPD molecular marker technology is a new efficient way to identify Pleurotus eryngii strains, and can be widely applied for classification and identification of edible mushroom.

Key words: Pleurotus eryngii, antagonistic effects, restriction endonuclease enzyme digestion, RF-RAPD (restriction fragment-random amplified polymeric DNA)